Entelechon receives BioChance Plus funding for a new method of protein quantification
The new method works for tan absolute quantification, which allows the multiplexed measurement of absolute protein levels of a large number of analytes in a single step. Such analytes can be retrieved from cell extracts or bodily fluids, and can cover proteomes or subproteomes. The method uses reference peptides – so-called peptide mass tags – which are encoded as a concatamar in a single gene, and which are produced biosynthetically by expresion of such a gene in a suitable host organism. By adding isotope-labelled metabolites, the reference peptides can easily be labelled, so as to differentiate between analyte and reference peptides. Both peptide fractions are generated at the same time in a single mixture, by adding the concatamer protein to the target protein solution and performing a sequence-specific proteolysis step. The peptide mixture can be used to quantify protein samples, for examples retrieved from tissue, with the help of mass spectrometry.
Possible applications of are quantitative proteomics in fundamental research, but also protein quantification in the development of diagnostics and within the drug discovery process, and routine testing in preclinical or toxicological settings.
Entelechon GmbH was founded in 1999 and produces custom synthetic genes and vectors. The company has developed a new synthesis method which enables Entelechon to rapidly perform gene assemblies at very low costs. Entelechon maintains a bioinformatics unit for the optimization and the support of the gene synthesis service, and is currently in the process of complementing its gene synthesis service with gene optimization software, protein quantification, and protein expression services.
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